Chromatogrophy

Purpose: In this lab experiment the mixtures of organic compounds are crystalized employ liquid chromatography. tender loving care separation takes rate on a thin layer of solid unmoving phase scattering on a solid alimentation as a liquid mobile phase, also patently called solvent, moves along the plate. In particular a Cartotenoid called Bixin is isolated from a mixture of Carotenoids put up in annatto seeds in this experiment. Although this proficiency is often use to separate uncolored mixtures of compounds in this vitrine colored compounds are used so that it is possible to surveil the separation as it occurs. Reagent submit: M.W(g)Molecular form Bixin394.5096C25H30O4 Methylbixin408.236C26H32O4 Norbixin380.216C24H28O4 All E-Geranylgeraniol288.194C20H32O Geranylgeraniol esters315.198C21H31O2 Phytoene541.4C40H64 Phytofluene542.896C40H60 Carotene536.88C40H56 Nurosprene538.864C40H58 cognitive operation: Place 2.0 grams of miserable annatto seeds in a 50 mL be aker. To the beaker furnish a magnetic advance barr and 5 mL of 8% neutral spirits in methylene chloride re dissolving agent. Allow the beginning to stir for 5 to 10 minutes. Vacuum filter the theme by a movement fritted filter. Transfer the dark chromatic filter out to a clean 50 mL beaker. Transfer the filtered solids tail into the original beaker.

Repeat the extraction of the crushed annatto seed residual with 5 mL of the 8% Ethanol in methylene chloride solution two more times. all(prenominal) time vacuum cleaner filter the orange solution through a course fritted funnel. Combine the annatto extract s into the same 50 mL beaker as the basic e! xtract. After completing the TLC analysis place a toiling chip in the annatto extract in the beaker and boil the solvent until only almost 1 mL remains. Do not boil the dryness. The solution spatters and bumps if boiled to dryness. Remove the be solvent by go across compressed air or nitrogen over... If you want to rush a full essay, golf club it on our website: BestEssayCheap.com

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